AI is transforming how transportation systems are designed, governed, and operated, presenting a prime vector for saving lives, time, and money. AI-enabled transportation improves roadway safety by predicting collisions, identifying hazardous conditions, and enabling faster emergency responses. However, despite these benefits, the U.S. Department of Transportation has only recently begun incorporating AI into its regulatory actions. AI in Motion analyzes AI applications and governance in transportation and lays out several guidelines for securing American leadership in and taking full advantage of AI-enabled transportation.